Paired-end sequencing allows users to sequence both ends of a fragment and generate high-quality, alignable sequence data. Paired-end sequencing . May 8, 2018 - Always easier to illustrate with an image, from here. The grey represents the fragment, and each end of the fragment is sequenced. This allows . Paired end and Mate pair Sequencing: What is it and How is it done? . Knowing the distance between each paired read helps to improve their specificity and . The library preparation for 'paired-end' sequencing, according to the lllumina technology, consists in fragmenting the genomic DNA mechanically (Covaris, . Paired end sequencing refers to the fact that the fragment(s) sequenced were sequenced from both ends and not just the one (as was true for first generation . The paired-end tag (PET) sequencing is one such strategy for improving DNA sequencing efficiency and enabling biological applications. In PET analysis, as . Dec 19, 2012 - In conventional paired-end sequencing, you simply sequence using the adapter for one end, and then once you're done you start over . Paired-end tags (PET) are the short sequences at the 5' and 3' ends of a DNA fragment which are unique enough that they (theoretically) exist together only once . Figure 8 Paired-end sequencing and alignment. Paired-end sequencing enables boths ends of the DNA fragment to be sequenced. Because the distance .